کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10841598 | 1067896 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Co-cultures of shoots and hairy roots of Genista tinctoria L. for synthesis and biotransformation of large amounts of phytoestrogens
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کلمات کلیدی
ABAIBAisoliquiritigeninindole-3-butyric acid - اسید Indole-3-Butyricabscisic acid - اسید آبسزیکBioconversion - تبدیل زیستیGenistin - جنیستینDaidzin - دلزینHairy roots - ریشه های مودارShoots - ساقه هاIn vitro cultures - فرهنگ های in vitrofresh weight - وزن ترdry weight - وزن خشکhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Co-cultures of shoots and hairy roots of Genista tinctoria L. for synthesis and biotransformation of large amounts of phytoestrogens Co-cultures of shoots and hairy roots of Genista tinctoria L. for synthesis and biotransformation of large amounts of phytoestrogens](/preview/png/10841598.png)
چکیده انگلیسی
Shoot and hairy root Genista tinctoria co-culture was established in Schenk and Hildebrandt medium supplemented with 24.6 μmol lâ1 indole-3-butyric acid (IBA) to produce large amounts of isoflavones of phytoestrogenic activity. The different tissue inoculation ratios were tested to achieve the best growth of G. tinctoria shoots and hairy roots in the co-culture system. In the co-culture, hairy roots produced large amounts of a single isoflavone-isoliquiritigenin (2473.8 mg/100 g dry weight (DW)), which is a daidzein precursor absent in the intact plant. Owing to the addition of abscisic acid (37.8 μmol lâ1-on day 42), isoliquiritigenin was almost completely released into the growth medium, from which it was used by the shoots to produce significant amounts of daidzin and daidzein. G. tinctoria shoots in a co-culture system, like in classic single cultures, maintained the ability to produce significant amounts of genistin (6941.5 mg/100 g DW) and its derivatives. At the same time, as a result of the described bioconversion of isoliquiritigenin, they synthesised 38 times more daidzin than the intact plant (1647.5 mg/100 g DW). This result suggests that G. tinctoria co-culture may be used to produce large amounts of isoflavone phytoestrogens. A prototype basket-bubble bioreactor was designed and built to upgrade the scale of the G. tinctoria co-culture. The new device significantly improved the growth parameters and the productivity of both tissues.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 169, Issue 5, November 2005, Pages 862-871
Journal: Plant Science - Volume 169, Issue 5, November 2005, Pages 862-871
نویسندگان
Maria Åuczkiewicz, Adam Kokotkiewicz,