Obtaining cellulose binding and hydrolyzing activity of a family 11 hybrid xylanase by fusion with xylan binding domain

► The ATx obtained cellulose binding and hydrolyzing activity by module shuffling. ► The ATxX was a bifunctional enzyme and possessed higher affinity for natural substrates. ► Xylan-binding domain and linker sequences played an important role in binding and hydrolyzing of xylanase to insoluble substrates.