A comparative analysis of rapid methods for purification and refolding of recombinant bovine prion protein

► Recombinant bovine prion purification methods based on metal affinity are compared. ► Purity, yield, and oxidation of various rPrP purification methods are directly compared. ► Recommendations for use of these small-scale preparations are provided. ► Findings are applicable to higher-throughput recombinant prion studies.