Cloning and constitutive expression of His-tagged xylanase GH 11 from Penicillium occitanis Pol6 in Pichia pastoris X33: Purification and characterization

► Isolation of the full-length cDNA of xylanase gene from Penicillium occitanis Pol6 using PCR approach. ► High-level constitutive expression of xylanase GH11 from P. occitanis Pol6 in Pichia pastoris. ► One-step purification protocol using immobilized metal affinity chromatography (Ni-NTA resin). ► Full characterization of the recombinant xylanase.