Reconstitution of the human chaperonin CCT by co-expression of the eight distinct subunits in mammalian cells

► We reconstituted chaperonin CCT, a 16-mer complex of 8 distinct subunits. ► The recombinant CCT was morphologically indistinguishable from the native CCT. ► The recombinant CCT was functionally indistinguishable from the native CCT.