Expression analysis of human β-secretase in transgenic tomato fruits

► Human β-secretase was successfully expressed in transgenic tomato fruit. ► We estimated the expression level of rBACE1 protein at 136 ± 7 ng mg−1 TSP. ► Tomato-derived rBACE1 retains its activity for a long storage period. ► Tomato-derived rBACE is severely degraded by heat or boiling. ► The proteolytic activity was similar to that of a commercial sample of Escherichia coli-derived BACE1.