Recombinant expression, purification and dimerization of the neurotrophic growth factor Artemin for in vitro and in vivo use

► A novel method for producing recombinant neurotrophic factor Artemin is described. ► Recombinant Artemin protein is dimeric, highly pure and active in a cellular assay. ► Dimerization and stability of Artemin are characterized using various methods. ► Experiments for development as therapeutic for neuropathic pain will be facilitated.