Characterization and identification of the protein partners of Fn3 domain in FnTm2

► Expression and purified of mammalian Fn3 protein domain with multiple disulfide bonds in a prokaryotic system. ► Fn3 domain refolding protocol and secondary structure analysis. ► In vitro pull-down assay using Fn3 to identify novel protein-protein interactions in bird brain. ► Confirmation of myelin basic protein binding to Fn3.