کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10843563 1069274 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Refolding and one-step purification of recombinant human ARA70 over-expressed in Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Refolding and one-step purification of recombinant human ARA70 over-expressed in Escherichia coli
چکیده انگلیسی
Androgen receptor (AR)-associated coregulator 70 (ARA70) is a cytoplasmic protein that has been characterized to have the ability to induce AR transcriptional activity in response to androgens and anti-androgens in prostate cancer cells. AR has been shown to have an important role in the progression of prostate cancer and in normal male reproductive system development. To elucidate the molecular mechanisms and biological relevance of ARA70 to prostrate cancer using a variety of biochemical analyses, the cDNA encoding full length ARA70 was cloned into pET21b vector. Here, we report the refolding and one-step purification of ARA70 from inclusion bodies over-expressed in Escherichia coli. The protein was purified to homogeneity, yielding ∼60 mg ARA70 from 1 L of terrific broth media. Refolding process of ARA70 was monitored using far-UV CD analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 39, Issue 2, February 2005, Pages 283-287
نویسندگان
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