کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10872912 1074253 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Activation of tyrosine kinase of EGFR induces Gβγ-dependent GRK-EGFR complex formation
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Activation of tyrosine kinase of EGFR induces Gβγ-dependent GRK-EGFR complex formation
چکیده انگلیسی
This study demonstrated that activation of tyrosine kinase of epidermal growth factor receptor (EGFR) induces its association with G protein-coupled receptor kinase 2 (GRK2). Immunoprecipitation experiments showed that EGF stimulation increased GRK2 binding to EGFR complex in HEK293 cells coexpressing EGFR and GRK2. The EGF-induced GRK2-EGFR complex formation was greatly reduced by perturbation of EGFR and Src tyrosine kinase activity. Furthermore, studies with GRK2 mutants showed that neither catalytic activity nor the N-terminal domain of GRK2 was required for EGF-induced GRK2-EGFR complex formation. However, overexpression of Gβγ scavengers blocked EGF-induced formation of GRK2-EGFR complex.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 1, 3 January 2005, Pages 122-126
نویسندگان
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