کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10889298 | 1080817 | 2009 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation requirements and responses to TLR ligands in human CD4+ T cells: Comparison of two T cell isolation techniques
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کلمات کلیدی
PhytohemagglutininIMACSTregPam3CysTLRIL-2IFN-γAPCTCrCPMPHAFACSPBMCmAbLPSMonoclonal antibody - آنتی بادی مونوکلونالAntigen presenting cells - آنتیژن ارائه سلولinterferon-gamma - اینترفرون گاماInterleukin-2 - اینترلوکین-۲ELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاToll-like receptor - تیالآرfluorescence-activated cell sorting - دسته بندی سلول های فعال فلورسنسRegulatory T cell - سلول T تنظیم کنندهperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیcounts per minute - شمار در هر دقیقهCellular activation - فعال سازی تلفن همراهlipopolysaccharide - لیپوپلی ساکاریدT cell receptor - گیرنده سلول TToll-like receptors - گیرنده های پولی مانند
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Activation requirements and responses to TLR ligands in human CD4+ T cells: Comparison of two T cell isolation techniques Activation requirements and responses to TLR ligands in human CD4+ T cells: Comparison of two T cell isolation techniques](/preview/png/10889298.png)
چکیده انگلیسی
Direct regulation of T cell function by microbial ligands through Toll-like receptors (TLR) is an emerging area of T cell biology. Currently either immunomagnetic cell sorting (IMACS) or fluorescence-activated cell sorting (FACS), are utilized to isolate T-cell subsets for such studies. However, it is unknown to what extent differences in T cell purity between these isolation techniques influence T cell functional assays. We compared the purity, response to mitogen, activation requirements, and response to TLR ligands between human CD4+ T cells isolated either by IMACS (IMACS-CD4+) or by IMACS followed by FACS (IMACS/FACS-CD4+). As expected, IMACS-CD4+ were less pure than IMACS/FACS-CD4+ (92.5% ± 1.4% versus 99.7% ± 0.2%, respectively). Consequently, IMACS-CD4+ proliferated and produced cytokines in response to mitogen alone and had lower activation requirements compared to IMACS/FACS-CD4+. In addition IMACS-CD4+ but not IMACS/FACS-CD4+ responses were upregulated by the TLR-4 ligand lipopolysaccharide (LPS). On the other hand, TLR-2 and TLR-5 engagement induced costimulation in both IMACS-CD4+ and highly purified IMACS-/FACS-CD4+. Altogether these results indicate that small differences in cell purity can significantly alter T cell responses to TLR ligands. This study stresses the importance of a stringent purification method when investigating the role of microbial ligands in T cell function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 344, Issue 1, 15 May 2009, Pages 15-25
Journal: Journal of Immunological Methods - Volume 344, Issue 1, 15 May 2009, Pages 15-25
نویسندگان
Christina L. Lancioni, Jeremy J. Thomas, Roxana E. Rojas,