An anion-exchange method to concentrate dissolved DNA from aquifer water

► We developed a rapid method to concentrate dissolved DNA (dDNA) from aquifer water. ► We tested dDNA stability in aquifer water and dDNA recovery parameters. ► We were able to PCR amplify 16S rRNA for T-RFLP and clone library analysis from aquifer dDNA. ► The ureC gene, broad-host-range IncP, IncQ and IncW plasmid genes were also detected from aquifer dDNA.