Evaluation of tyramide solutions for an improved detection and enumeration of single microbial cells in soil by CARD-FISH

► Tyramide solutions for the application of CARD-FISH in soil were evaluated. ► Self-synthesized tyramides superior to commercially available products ► Highly reproducible cell numbers and detection rates in different soils ► Reliable detection and quantification of single microbial cells in soil ► Positive effects of IPBA and NaCl on CARD-FISH