Development and evaluation of an enzyme-linked immunosorbent assay based on a recombinant SpaA protein (rSpaA415) for detection of anti-Erysipelothrix spp. IgG antibodies in pigs

► An Erysipelothrix spp. ELISA based on the SpaA protein was developed. ► The ELISA was found to be specific and sensitive on sera from experimentally-infected pigs. ► Earliest detection of anti-Erysipelothrix IgG antibodies was 7 days post exposure. ► Cross-reaction with antisera against Erysipelothrix tonsillarum was not observed.