کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10899724 | 1084401 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ribosomal protein S3 regulates GLI2-mediated osteosarcoma invasion
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
SDSglioma-associated oncogene homologrps3Patched1Gli2SMOPTCH1GliTBSTSufuDMEMFBSATODulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoRNA interference - RNA تداخل کنندهRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهβ-actin - β-اکتینarsenic trioxide - آرسنیک تریاکسیدOsteosarcoma - استئوسارکوماTris-buffered saline Tween-20 - تریس بافر Tween-20 سالینHedgehog - جوجهتیغی، خارپشتsodium dodecyl sulfate - سدیم دودسیل سولفاتfetal bovine serum - سرم جنین گاوsuppressor of fused - سرکوب کننده همجوشیSmoothened - صافActb - عملRibosomal protein S3 - پروتئین Ribosomal S3
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
It has been reported that GLI2 promotes proliferation, migration, and invasion of mesenchymal stem cell and osteosarcoma cells. To examine the molecular mechanisms of GLI2-mediated osteosarcoma metastasis, we performed a microarray analysis. The gene encoding ribosomal protein S3 (RPS3) was identified as a target of GLI2. Real-time PCR revealed that RPS3 was upregulated in osteosarcoma cell lines compared with normal osteoblast cells. Knockdown of GLI2 decreased RPS3 expression, whereas forced expression of a constitutively active form of GLI2 upregulated the expression of RPS3. RPS3 knockdown by siRNA decreased the migration and invasion of osteosarcoma cells. Although forced expression of constitutively active GLI2 increased the migration of human mesenchymal stem cells, knockdown of RPS3 reduced the up-regulated migration. In contrast, forced expression of RPS3 increased migration and invasion of osteosarcoma cells. Moreover, reduction of migration by GLI2 knockdown was rescued by forced expression of RPS3. Immunohistochemical analysis showed that RPS3 expression was increased in primary osteosarcoma lesions with lung metastases compared with those without. These findings indicate that GLI2-RPS3 signaling may be a marker of invasive osteosarcoma and a therapeutic target for patients with osteosarcoma.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 356, Issue 2, Part B, 28 January 2015, Pages 855-861
Journal: Cancer Letters - Volume 356, Issue 2, Part B, 28 January 2015, Pages 855-861
نویسندگان
Hiroko Nagao-Kitamoto, Takao Setoguchi, Sho Kitamoto, Shunsuke Nakamura, Arisa Tsuru, Masahito Nagata, Satoshi Nagano, Yasuhiro Ishidou, Masahiro Yokouchi, Shinichi Kitajima, Takako Yoshioka, Shingo Maeda, Suguru Yonezawa, Setsuro Komiya,