کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10903714 | 1086513 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
βâTaxilin participates in differentiation of C2C12 myoblasts into myotubes
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کلمات کلیدی
SNXGFPMyogenesisGAPDHC2C12VAMPDMEMDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAdifferentiation medium - تمایز رسانهDysbindin - دیسیندینreverse transcription - رونویسی معکوسMyosin heavy chain - زنجیره سنگین میوزینMHC - مجموعه سازگاری بافتی اصلیSorting nexin - مرتب سازی ~~ V نهVesicle-associated membrane protein - پروتئین غشاء مرتبط با Vesiclegreen fluorescent protein - پروتئین فلورسنت سبزglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازtransferrin receptor - گیرنده انتقالین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: βâTaxilin participates in differentiation of C2C12 myoblasts into myotubes βâTaxilin participates in differentiation of C2C12 myoblasts into myotubes](/preview/png/10903714.png)
چکیده انگلیسی
Myogenesis is required for the development of skeletal muscle. Accumulating evidence indicates that the expression of several genes are upregulated during myogenesis and these genes play pivotal roles in myogenesis. However, the molecular mechanism underlying myogenesis is not fully understood. In this study, we found that β-taxilin, which is specifically expressed in the skeletal muscle and heart tissues, was progressively expressed during differentiation of C2C12 myoblasts into myotubes, prompting us to investigate the role of β-taxilin in myogenesis. In C2C12 cells, knockdown of β-taxilin impaired the fusion of myoblasts into myotubes, and decreased the diameter of myotubes. We also found that β-taxilin interacted with dysbindin, a coiled-coil-containing protein. Knockdown of dysbindin conversely promoted the fusion of myoblasts into myotubes and increased the diameter of myotubes in C2C12 cells. Furthermore, knockdown of dysbindin attenuated the inhibitory effect of β-taxilin depletion on myotube formation of C2C12 cells. These results demonstrate that β-taxilin participates in myogenesis through suppressing the function of dysbindin to inhibit the differentiation of C2C12 myoblasts into myotubes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 345, Issue 2, 15 July 2016, Pages 230-238
Journal: Experimental Cell Research - Volume 345, Issue 2, 15 July 2016, Pages 230-238
نویسندگان
Hiroshi Sakane, Tomohiko Makiyama, Satoru Nogami, Yukimi Horii, Kenji Akasaki, Hiromichi Shirataki,