کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10903907 | 1086539 | 2015 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DEAD-box helicase DDX27 regulates 3â² end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: DEAD-box helicase DDX27 regulates 3â² end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex DEAD-box helicase DDX27 regulates 3â² end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex](/preview/png/10903907.png)
چکیده انگلیسی
PeBoW, a trimeric complex consisting of pescadillo (Pes1), block of proliferation (Bop1), and the WD repeat protein 12 (WDR12), is essential for processing and maturation of mammalian 5.8S and 28S ribosomal RNAs. Applying a mass spectrometric analysis, we identified the DEAD-box helicase DDX27 as stably associated factor of the PeBoW-complex. DDX27 interacts with the PeBoW-complex via an evolutionary conserved FÃF motif in the N-terminal domain and is recruited to the nucleolus via its basic C-terminal domain. This recruitment is RNA-dependent and occurs independently of the PeBoW-complex. Interestingly, knockdown of DDX27, but not of Pes1, induces the accumulation of an extended form of the primary 47S rRNA. We conclude that DDX27 can interact specifically with the Pes1 and Bop1 but fulfils critical function(s) for proper 3â² end formation of 47S rRNA independently of the PeBoW-complex.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 334, Issue 1, 15 May 2015, Pages 146-159
Journal: Experimental Cell Research - Volume 334, Issue 1, 15 May 2015, Pages 146-159
نویسندگان
Markus Kellner, Michaela Rohrmoser, Ignasi Forné, Kirsten Voss, Kaspar Burger, Bastian Mühl, Anita Gruber-Eber, Elisabeth Kremmer, Axel Imhof, Dirk Eick,