کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10903972 | 1086544 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Potential effect of matrix stiffness on the enrichment of tumor initiating cells under three-dimensional culture conditions
ترجمه فارسی عنوان
اثر پتانسیل سختی ماتریکس بر غنی سازی سلولهای آغاز کننده تومور در شرایط کشت سه بعدی
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کلمات کلیدی
فرهنگ سه بعدی، دانه های آلژینات ژل، تومور سلولهای آغازین، سختی ماتریس،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
چکیده انگلیسی
Cancer stem cell (CSC) or tumor initiating cell (TIC) plays an important role in tumor progression and metastasis. Biophysical forces in tumor microenvironment have an important effect on tumor formation and development. In this study, the potential effect of matrix stiffness on the biological characteristics of human head and neck squamous cell carcinoma (HNSCC) TICs, especially the enrichment of HNSCC TICs, was investigated under three-dimensional (3D) culture conditions by means of alginate gel (ALG) beads with different matrix stiffnesses. ALG beads with soft (21Â kPa), moderate (70Â kPa) and hard (105Â kPa) stiffness were generated by changing alginate concentration. It was found that significant HNSCC TIC enrichment was achieved in the ALG beads with moderate matrix stiffness (70Â kPa). The gene expression of stemness markers Oct3/4 and Nanog, TIC markers CD44 and ABCG2 was enhanced in cells under this moderate (70Â kPa) stiffness. HNSCC TIC proportion was also highly enriched under moderate matrix stiffness, accompanying with higher tumorigenicity, metastatic ability and drug resistance. And it was also found that the possible molecular mechanism underlying the regulated TIC properties by matrix stiffness under 3D culture conditions was significantly different from 2D culture condition. Therefore, the results achieved in this study indicated that 3D biophysical microenvironment had an important effect on TIC characteristics and alginate-based biomimetic scaffolds could be utilized as a proper platform to investigate the interaction between tumor cells and 3D microenvironment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 330, Issue 1, 1 January 2015, Pages 123-134
Journal: Experimental Cell Research - Volume 330, Issue 1, 1 January 2015, Pages 123-134
نویسندگان
Chang Liu, Yang Liu, Xiao-xi Xu, Hao Wu, Hong-guo Xie, Li Chen, Ting Lu, Li Yang, Xin Guo, Guang-wei Sun, Wei Wang, Xiao-jun Ma, Xin He,