α-Actinin1 and 4 tyrosine phosphorylation is critical for stress fiber establishment, maintenance and focal adhesion maturation

► α-Actinin 1/4 is required for actin stress fiber establishment and maintenance. ► α-Actinin 1/4 alters focal adhesions by affecting mobilization of Zyxin and VASP. ► Tyrosine phosphorylation of a-actinin 1/4 is critical for these functions.