کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10904444 | 1086592 | 2012 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CD45lowc-Kithigh cells have hematopoietic properties in the mouse aorta-gonad-mesonephros region
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: CD45lowc-Kithigh cells have hematopoietic properties in the mouse aorta-gonad-mesonephros region CD45lowc-Kithigh cells have hematopoietic properties in the mouse aorta-gonad-mesonephros region](/preview/png/10904444.png)
چکیده انگلیسی
Long-term reconstituting hematopoietic stem cells first arise from the aorta of the aorta-gonad-mesonephros (AGM) region in a mouse embryo. We have previously reported that in cultures of the dispersed AGM region, CD45lowc-Kit+ cells possess the ability to reconstitute multilineage hematopoietic cells, but investigations are needed to show that this is not a cultured artifact and to clarify when and how this population is present. Based on the expression profile of CD45 and c-Kit in freshly dissociated AGM cells from embryonic day 9.5 (E9.5) to E12.5 and aorta cells in the AGM from E13.5 to E15.5, we defined six cell populations (CD45âc-Kitâ, CD45âc-Kitlow, CD45âc-Kithigh, CD45lowc-Kithigh, CD45highc-Kithigh, and CD45highc-Kitvery low). Among these six populations, CD45lowc-Kithigh cells were most able to form hematopoietic cell colonies, but their ability decreased after E11.5 and was undetectable at E13.5 and later. The CD45lowc-Kithigh cells showed multipotency in vitro. We demonstrated further enrichment of hematopoietic activity in the Hoechst dye-effluxing side population among the CD45lowc-Kithigh cells. Here, we determined that CD45lowc-Kithigh cells arise from the lateral plate mesoderm using embryonic stem cell-derived differentiation system. In conclusion, CD45lowc-Kithigh cells are the major hematopoietic cells of mouse AGM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 318, Issue 6, 1 April 2012, Pages 705-715
Journal: Experimental Cell Research - Volume 318, Issue 6, 1 April 2012, Pages 705-715
نویسندگان
Ikuo Nobuhisa, Shoutarou Yamasaki, Ahmed Ramadan, Tetsuya Taga,