کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10904475 | 1086593 | 2012 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Establishment and characterization of mouse bone marrow-derived mast cell hybridomas
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کلمات کلیدی
DNP-HSADNPGM-CSFFcεRIBMMCGAPDHIgEFITChypoxanthine–aminopterin–thymidineFBSCOXLPSBSA - BSADMSO - DMSObovine serum albumin - آلبومین سرم گاوcyclooxygenase - آنزیم سیکلواکسیژنازImmunoglobulin E - ایمونوگلوبولین Einterleukin - اینترلوکینdinitrophenol - دینیتروفنلDimethyl sulfoxide - دیمتیل سولفواکسیدfetal bovine serum - سرم جنین گاوgranulocyte-macrophage colony-stimulating factor - عامل گرانولوسیت-ماکروفاژ colony-stimulating factorfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتLeukotriene - لکوترینlipopolysaccharide - لیپوپلی ساکاریدHAT - کلاهglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازhigh-affinity IgE receptor - گیرنده IgE باکتری
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
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چکیده انگلیسی
Interleukin (IL)-3-dependent mouse bone marrow-derived mast cells (BMMCs) are an important model for studying the function of mucosal-type mast cells. In the present study, BMMCs were successfully immortalized by cell fusion using a hypoxanthine-aminopterin-thymidine medium-sensitive variant of P815 mouse mastocytoma (P815-6TgR) as a partner cell line. The established mouse mast cell hybridomas (MMCHs) expressed α, β, and γ subunits of high-affinity immunoglobulin E (IgE) receptor (FcεRI) and possessed cytoplasmic granules devoid of or partially filled with electron-dense material. Four independent MMCH clones continuously proliferated without supplemental exogenous IL-3 and showed a degranulation response on stimulation with IgE+antigen. Furthermore, histamine synthesis and release by degranulation were confirmed in MMCH-D5, a MMCH clone that showed the strongest degranulation response. MMCH-D5 exhibited elevated levels of IL-3, IL-4, IL-13, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor (TNF)-α, and cyclooxygenase 2, and production of prostaglandin D2 and leukotriene C4 in response to IgE-induced stimulation. MMCH clones also expressed Toll-like receptors (TLRs) 1, 2, 4, and 6 and showed elevated levels of TNF-α expression in response to stimulation with TLR2 and TLR4 ligands. The MMCHs established using this method should be suitable for studies on FcεRI- and TLR-mediated effector functions of mast cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 318, Issue 18, 1 November 2012, Pages 2385-2396
Journal: Experimental Cell Research - Volume 318, Issue 18, 1 November 2012, Pages 2385-2396
نویسندگان
Takeshi Kawahara,