کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10905459 1086756 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reversible and repeatable linear local cell force response under large stretches
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Reversible and repeatable linear local cell force response under large stretches
چکیده انگلیسی
Large stretching and un-stretching force response of adherent fibroblasts is measured by micromachined mechanical force sensors. The force sensors are composed of a probe and flexible beams. The probe, functionalized by fibronectin, is used to contact the cells. The flexible beams are the sensing element. The sensors are made of single crystal silicon and fabricated by the SCREAM process. The maximum cell stretch reached is ∼50 μm, which is about twice of the cell initial size, and the time delay between two consecutive stretching/un-stretching steps is 75 s unless otherwise stated. We find that the force response of the cells is strongly linear, reversible, and repeatable, with a small stiffening at the initial deformation stage. Force response of single cells measured before and after cytochalasin D treatment suggests that actin filaments take almost all the cell internal forces due to stretch. These findings may shed light on the increasing understanding on the mechanical behavior of cells and provide clues for making new classes of biological materials having uncommon properties.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 305, Issue 1, 15 April 2005, Pages 42-50
نویسندگان
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