کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10956188 | 1098785 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential involvement of signaling pathways in the regulation of growth hormone release by somatostatin and growth hormone-releasing hormone in orange-spotted grouper (Epinephelus coioides)
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کلمات کلیدی
U73122GHRHGH releaseGPCRGH-releasing hormonePLCdiC8PKCSSTRSGCPACAPH891,2-dioctanoyl-sn-glycerolGF109203XSRIFGHRH receptorS-nitroso-N-acetylpenicillamineGnRHPhorbol 12-myristate-13-acetateFBSPBSNOSpKacGMPtPABSA - BSAcAMP - cAMPG-protein-coupled receptor - G-پروتئین گیرندهguanosine 3′,5′-cyclic monophosphate - guanosine 3 '، 5'-cyclic monophosphateinositol phosphate - inositol فسفاتl-NAME - L-NAMEMAPK - MAPKadenosine 3′,5′-cyclic monophosphate - آدنوزین 3 '، 5'-سیکلیک منوفسفرهadenylate cyclase - آدنیلات سیکلاسbovine serum albumin - آلبومین سرم گاوDopamine - دوپامینdiacylglycerol - دیسیل گلیسیرینDAG - روزfetal bovine serum - سرم جنین گاوSomatostatin - سوماتواستاتینSoluble guanylate cyclase - سیکلاس گوانیلات حل کنندهSNAP - ضربه محکم و ناگهانیPhosphate buffered saline - فسفات بافر شورphospholipase C - فسفولیپاز CSignaling pathway - مسیر سیگنالینگNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازGonadotropin-releasing hormone - هورمون آزاد کننده گنادوتروپینGrowth hormone - هورمون رشدprotein kinase A - پروتئین کیناز AProtein kinase C - پروتئین کیناز سیmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenpituitary adenylate cyclase-activating peptide - پپتید فعال فعال آدنیلات سیکلاس هیپوفیزSomatostatin receptor - گیرنده سوماتوستاتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Somatostatin is the most effective inhibitor of GH release, and GHRH was recently identified as one of the primary GH-releasing factors in teleosts. In this study, we analyzed the possible intracellular transduction pathways that are involved in the mechanisms induced by SRIF and GHRH to regulate GH release. Using a pharmacological approach, the blockade of the PLC/IP/PKC pathway reversed the SRIF-induced inhibition of GH release but did not affect the GHRH-induced stimulation of GH release. Furthermore, SRIF reduced the GH release induced by two PKC activators. Inhibitors of the AC/cAMP/PKA pathway reversed both the SRIF- and GHRH-induced effects on GH release. Moreover, the GH release evoked by forskolin and 8-Br-cAMP were completely abolished by SRIF. The blockade of the NOS/NO pathway attenuated the GHRH-induced GH release but had minimal effects on the inhibitory actions of SRIF. In addition, inhibitors of the sGC/cGMP pathway did not modify the SRIF- or GHRH-induced regulation of GH release. Taken together, these findings indicate that the SRIF-induced inhibition of GH release is mediated by both the PLC/IP/PKC and the AC/cAMP/PKA pathways and not by the NOS/NO/sGC/cGMP pathway. In contrast, the GHRH-induced stimulation of GH secretion is mediated by both the AC/cAMP/PKA and the NOS/NO pathways and is independent of the sGC/cGMP pathway and the PLC/IP/PKC system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 382, Issue 2, 15 February 2014, Pages 851-859
Journal: Molecular and Cellular Endocrinology - Volume 382, Issue 2, 15 February 2014, Pages 851-859
نویسندگان
Bin Wang, Chaobin Qin, Cong Zhang, Jirong Jia, Caiyun Sun, Wensheng Li,