کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10962425 1102635 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimization and scale-up of cell culture and purification processes for production of an adenovirus-vectored tuberculosis vaccine candidate
ترجمه فارسی عنوان
بهینه سازی و افزایش تولید سلول ها و فرآیندهای تصفیه برای تولید یک کاندید واکسن سل ریوی عودنوویروس
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
چکیده انگلیسی
The AdAg85A generated from the 3 L and 60 L bioreactor runs was purified through several purification steps. More than 98% of total cellular proteins was removed, over 60% of viral particles was recovered after the purification process, and purity of AdAg85A was similar to that of the ATCC VR-1516 Ad5 standard. Vaccination of mice with the purified AdAg85A demonstrated a very good level of Ag85A-specific antibody responses. The optimized production and purification conditions were transferred to a GMP facility for manufacturing of AdAg85A for generation of clinical grade material to support clinical trials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 34, Issue 29, 17 June 2016, Pages 3381-3387
نویسندگان
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