کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10962446 | 1102636 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Core bead chromatography for preparation of highly pure, infectious respiratory syncytial virus in the negative purification mode
ترجمه فارسی عنوان
کروماتوگرافی باند هسته ای برای تهیه ویروس سونسیتیال تنفسی بسیار خالص و عفونی در حالت تصفیه منفی
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کلمات کلیدی
ویروس سونسیتیال تنفسی، تصفیه ویروس، هسته کروماتوگرافی مهره، فیلتر فیبر توخالی فیلتراسیون جریان مماسی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
چکیده انگلیسی
Respiratory syncytial virus (RSV) is an important human pathogen, and is the most frequent viral cause of severe respiratory disease in infants. In addition, it is increasingly being recognized as an important cause of respiratory disease in the elderly and immunocompromised. Although a passive prophylactic treatment does exist for high-risk neonates and children, the overall disease burden warrants the development of a safe and effective prophylactic vaccine for use in otherwise healthy newborns and children. RSV is known to be an extremely labile virus, prone to aggregation and loss of infectious titer during virus handling and preparation procedures. To date infective RSV virions have been prepared by methods which are not readily scalable, such as density gradient ultracentrifugation. In this study we describe a scalable, chromatography-based purification procedure for preparation of highly pure, infectious RSV. The purification scheme is based on core bead technology and hollow fiber tangential flow filtration (TFF) and results in a â¼60% recovery of infectious virus titer. This method can be used to prepare highly purified wild type or live-attenuated vaccine strain viruses with titers as high as 1Â ÃÂ 108 plaque forming units per mL. A live-attenuated RSV vaccine prepared by this method was found to be immunogenic and protective in vivo, and its purity was 50-200-fold greater with respect to host cell dsDNA and Vero host cell proteins, than the raw feed stream. The results presented here can be considered a starting point for downstream process development of a live-attenuated vaccine approach for prevention of disease by RSV.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 34, Issue 32, 12 July 2016, Pages 3690-3696
Journal: Vaccine - Volume 34, Issue 32, 12 July 2016, Pages 3690-3696
نویسندگان
Sophia T. Mundle, Michael Kishko, Rachel Groppo, Joshua DiNapoli, John Hamberger, Bryan McNeil, Harry Kleanthous, Mark Parrington, Linong Zhang, Stephen F. Anderson,