کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10963275 | 1102676 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Successful cross-protective efficacy induced by heat-adapted live attenuated nephropathogenic infectious bronchitis virus derived from a natural recombinant strain
ترجمه فارسی عنوان
اثربخشی موفقیت آمیز محافظت محافظتی ناشی از ویروس برونشیت عفونی ناشی از گرگرفتگی ملایم که از یک سوند طبیعی نوترکیب طبیعی
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کلمات کلیدی
ویروس برونشیت عفونی، انطباق حرارتی، تضعیفی، ایمنی، اثربخشی محافظتی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
چکیده انگلیسی
A natural recombinant nephropathogenic K40/09 strain of infectious bronchitis virus (IBV) was heat-adapted for possible future use as live attenuated vaccine. The K40/09 strain was selected during successive serial passages in specific-pathogen free (SPF) embryonated eggs at sub-optimal higher temperature (56 °C). Unlike the parental strain, the attenuated strain, designated K40/09 HP50, was found to be safe in 1-day-old SPF chicks, which showed neither mortality nor signs of morbidity, and rarely induced ciliostasis or histological changes in the trachea and kidney after intraocular and fine-spray administration. K40/09 HP50 provided almost complete protection against two distinct subgroups of a nephropathogenic strain (KM91-like and QX-like subgroup) and elicited the production of high titers of neutralizing antibody (neutralization index of 3.6). We conclude that the K40/09 HP50 vaccine virus is rapidly attenuated by heat adaptation and exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for a live attenuated vaccine. These results indicate that the K40/09 vaccine could be helpful for the reduction of economic losses caused by recently emergent nephropathogenic IBV infection in many countries.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 33, Issue 51, 16 December 2015, Pages 7370-7374
Journal: Vaccine - Volume 33, Issue 51, 16 December 2015, Pages 7370-7374
نویسندگان
Tae-Hyun Lim, Ha-Na Youn, Seong-Su Yuk, Jung-Hoon Kwon, Woo-Tack Hong, Gyeong-Bin Gwon, Jung-Ah Lee, Joong-Bok Lee, Sang-Won Lee, Chang-Seon Song,