Application of Bluetongue Disabled Infectious Single Animal (DISA) vaccine for different serotypes by VP2 exchange or incorporation of chimeric VP2

- VP2 from several serotypes can be expressed on bluetongue virus DISA vaccine.
- Exchange of VP2 from some serotypes on bluetongue virus DISA vaccine is not possible.
- Chimeric VP2 of BTV1 and BTV16 is functional in vitro and in vivo.
- BTV with chimeric VP2 is neutralized by sera against both parental serotypes.
- BTV DISA vaccine with chimeric VP2 induces nAbs against both parental serotypes.