کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10963794 | 1102693 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunogenicity of porcine P[6], P[7]-specific â³VP8* rotavirus subunit vaccines with a tetanus toxoid universal T cell epitope
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Immunogenicity of porcine P[6], P[7]-specific â³VP8* rotavirus subunit vaccines with a tetanus toxoid universal T cell epitope Immunogenicity of porcine P[6], P[7]-specific â³VP8* rotavirus subunit vaccines with a tetanus toxoid universal T cell epitope](/preview/png/10963794.png)
چکیده انگلیسی
Currently, commercial porcine rotavirus vaccines remain varied limitations. The objective of this study is to develop an alternative porcine rotavirus subunit vaccine candidate by parenteral administration, which enables to elicit robust immune responses against most prevalence porcine rotavirus strains. The bacterially-expressed porcine rotavirus P[6]- or P[7]-specific truncated VP8* (aa 64-223) recombinant protein with or without a universal tetanus toxoid CD4+ T cell epitope P2 was generated. All the recombinant subunit proteins â³VP8*s or P2-â³VP8*s were of high solubility and high yields. The immunogenicity of each purified â³VP8* and P2-â³VP8* was evaluated in mice (10 μg/dose) or guinea pigs (20 μg/dose) immunized IM with 600 μg aluminum hydroxide three times at 2-week interval. The introduction of P2T cell epitope to P[7]-â³VP8* elicited significantly higher IgG titer in mice than its absence. Comparatively, P2 epitope slightly enhanced the immunogenicity of P[6]-â³VP8*. P2-P[7]â³VP8* elicited high titer of neutralizing antibody against heterotypic P[7]-specific rotaviruses with varied G type combination. Our data indicated that two subunit vaccines could be plausible bivalent rotavirus vaccine candidate to provide antigenic coverage of porcine rotavirus strains of global or regional importance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 33, Issue 36, 26 August 2015, Pages 4533-4539
Journal: Vaccine - Volume 33, Issue 36, 26 August 2015, Pages 4533-4539
نویسندگان
Xiaobo Wen, Xiaoman Wei, Xuhua Ran, Hongbo Ni, Si Cao, Yao Zhang,