کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10964587 | 1102721 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rescue of CD8+ T cell vaccine memory following sublethal γ irradiation
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کلمات کلیدی
qPCRCTLOVALLOIrradiated - تابش شدchicken ovalbumin - تخم مرغ تخم مرغیDAY - روزcytotoxic T lymphocyte - لنفوسیت T سیتوتوکسیکListeria monocytogenes - لیستریا مونوسیتوژنزListeriolysin O - لیستریولیزین Oquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیPost-irradiation - پس از تابش
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Rescue of CD8+ T cell vaccine memory following sublethal γ irradiation Rescue of CD8+ T cell vaccine memory following sublethal γ irradiation](/preview/png/10964587.png)
چکیده انگلیسی
Sublethal γ irradiation eliminates CD8+ T cell mediated memory responses. In this work, we explored how these memory responses could be rescued in the aftermath of such exposure. We utilized two models of CD8+ T cell mediated immunity: a mouse model of Listeria monocytogenes (LM) infection in which CD8+ T cells specific for LM expressed antigens (Listeriolysin O, LLO) can be tracked, and a murine skin graft model in which CD8+ T cells mediate rejection across a MHC class I (Dd) disparity. In the LM immunized mice, LL0 specific CD8+ T memory cells were lost on irradiation, preserved with rapid revaccination with an attenuated strain 1-3 days post-irradiation (PI), and these mice survived a subsequent wild type LM challenge. A genetic “signature of rescue” identified a group of immune-associated mRNA maintained or upregulated following irradiation and rescue. A number of these factors, including IL-36γ, dectin-2 (Clec4n), and mir101c are upregulated rapidly after exposure of mice to sublethal γ radiation alone and are sustained by early, but not later rescue. Such factors will be evaluated as potential therapeutics to replace individual vaccines for global rescue of CD8+ T memory cell responses following sublethal γ irradiation. The skin allograft model mirrored that of the LM model in that the accelerated Dd skin allograft rejection response was lost in mice exposed to sublethal γ radiation, but infusion of allogeneic Dd expressing bone marrow cells 1-4 days PI preserved the CD8+ T memory mediated accelerated rejection response, further suggesting that innate immune responses may not always be essential to rescue of CD8+ memory T cells following γ irradiation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 33, Issue 32, 31 July 2015, Pages 3865-3872
Journal: Vaccine - Volume 33, Issue 32, 31 July 2015, Pages 3865-3872
نویسندگان
Hugh I. McFarland, Julia D. Berkson, Jay P. Lee, Abdel G. Elkahloun, Karen P. Mason, Amy S. Rosenberg,