کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10965438 | 1102748 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A new recombinant SsnA protein combined with aluminum hydroxide protects mouse against Streptococcus suis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
An experimental challenge in a mouse model was used to select the most effective adjuvant in a vaccine formulation with the surface-anchored DNA-nuclease (SsnA). We used a protocol based on clinical, histopathological, bacterial kinetics and immune response against S. suis serotype 2 in infected animals. The three adjuvants used, aluminum hydroxide (ALOH), incomplete Freund's adjuvant (FIA), oil-in-water adjuvant (OW) showed a protective effect against death by S. suis serotype 2 in this mouse model, although aluminum hydroxide revealed as the best option. Subsequently, in a second experimental assay, we showed that a recombinant SsnA protein combined with ALOH as adjuvant allowed a significant decrease of clinical and lesional findings in animals, faster reduction of the bacteria from organs and a highest humoral response against S. suis after 3 days post-infection. The results show that this combination (rSsnAÂ +Â AlOH) could be a good vaccine formulation against S. suis, although further studies are necessary to evaluate their use for swine and human species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 32, Issue 51, 5 December 2014, Pages 6992-6999
Journal: Vaccine - Volume 32, Issue 51, 5 December 2014, Pages 6992-6999
نویسندگان
Lidia Gómez-Gascón, Fernando Cardoso-Toset, Paola Shyrley Amarilla, Carmen Tarradas, Librado Carrasco, Alfonso Olaya-Abril, Irene Jiménez-MunguÃa, Manuel J. RodrÃguez-Ortega, Inmaculada Luque,