کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10965842 | 1102761 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Recombinant duck enteritis virus expressing the HA gene from goose H5 subtype avian influenza virus
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کلمات کلیدی
CPECEFDEFdevHPAIVglycoprotein IDuck enteritis virus (DEV)Post-vaccinationcytopathic effects - اثرات سیتوپاتیکChicken embryo fibroblast - فیبروبلاست جنین مرغhemagglutinin - هماگلوتینینhemagglutinin (HA) - هموگلوتینین (HA)Duck enteritis virus - ویروس آندروتیک اردکhighly pathogenic avian influenza virus - ویروس آنفولانزای مرغی بسیار بیماریزای ویروس استunique short - کوتاه منحصر به فردGlycoprotein E - گلیکوپروتئین E
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The duck enteritis virus (DEV) may be a promising candidate viral vector for an aquatic poultry vaccination that can protect against multiple pathogens because it has a very large genome and a narrow host range. Recently, we described two DEV recombinants that contained deletions of the viral US2 or gIgE genes. The hemagglutinin (HA) gene of an H5N1-type highly pathogenic avian influenza virus (HPAIV) of goose origin was inserted into the deletion sites to construct two rDEVs expressing the AIV HA antigen. The resulting rDEV-ÎgIgE-HA or rDEV-ÎUS2-HA recombinant DEV viruses were used to infect duck embryo fibroblasts. Reverse transcription PCR, immunofluorescence and western blot analysis results indicated that rDEV-ÎgIgE-HA and rDEV-ÎUS2-HA were successfully expressed in duck embryo fibroblasts (DEFs). To investigate whether the HA gene could be stably maintained in the recombinant viruses, the viruses were passaged in DEFs 18 times. The HA gene in both recombinants could be detected by PCR amplification. The immunized four-week-old ducks induced specific antibodies against DEV and AIV HA and were protected against challenge infections with DEV AV1221 viruses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 31, Issue 50, 5 December 2013, Pages 5953-5959
Journal: Vaccine - Volume 31, Issue 50, 5 December 2013, Pages 5953-5959
نویسندگان
Xiaomei Liu, Shuangshi Wei, Yan Liu, Peifen Fu, Mingchun Gao, Xiaoyu Mu, Hua Liu, Mingwei Xing, Bo Ma, JunWei Wang,