کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10965984 | 1102765 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Control of antigen-binding to aluminum adjuvants and the immune response with a novel phosphonate linker
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کلمات کلیدی
3-(N-morpholino)propane-sulfonic acidAluminum phosphate adjuvantNHSPBS-TRPAAluminum adjuvantN-hydroxysuccinimideMOPSAluminum hydroxide adjuvant - آلژینوم هیدروکسید adjuvantRecombinant protective antigen - آنتی ژن محافظت کنندهhen egg lysozyme - تخم مرغ لیزوزیمdiphtheria toxoid - توکسوئیدی دیفتریtetanus toxoid - توکسوئین تانتانسAdsorption - جذبPhosphate - فسفاتLigand exchange - مبادله لیگاندIsoelectric point - نقطه ایزوالکتریکHEL - کامل
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The strongest mechanism for adsorption of antigens to aluminum adjuvants is ligand exchange, which involves the replacement of a surface hydroxyl on the adjuvant by a terminal phosphate group of the antigen. A novel phosphonate linker was developed that allows the addition of phosphonate (C-PO3) groups to proteins under controlled and chemically mild conditions. Increasing the number of linkers per protein molecule progressively increased the adsorption strength to aluminum hydroxide adjuvant (AH) as measured by elution in serum. The effect of phosphonate conjugation on the antibody response was determined with hen egg lysozyme (HEL), a protein that has the same charge as AH at neutral pH and does not adsorb to AH. The phosphonylated form of HEL (HEL-P) adsorbed to AH, indicating that the ligand exchange interaction could overcome the electrostatic repulsion. Mice injected with HEL-P/AH had a higher antibody titer to HEL than mice injected with HEL/AH, especially at lower antigen doses, suggesting that adsorption of antigen has a dose-sparing effect. Conjugation of CRM197, an antigen that adsorbs electrostatically to AH, with phosphonate linkers did not enhance the antibody response, indicating that adsorption by either electrostatic or ligand exchange to AH is sufficient to enhance the antibody response.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 31, Issue 40, 13 September 2013, Pages 4362-4367
Journal: Vaccine - Volume 31, Issue 40, 13 September 2013, Pages 4362-4367
نویسندگان
Fangjia Lu, Irene Boutselis, Richard F. Borch, Harm HogenEsch,