کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10966415 | 1102777 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pneumococcal polysaccharide vaccination induces polysaccharide-specific B cells in adult peripheral blood expressing CD19+CD20+CD3âCD70âCD27+IgM+CD43+CD5+/â
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کلمات کلیدی
PPVOPTFMOCD43TI-2MZBPps - PPSantibodies - آنتی بادی هاStreptococcus pneumoniae - استرپتوکوک پنومونیهfluorescence minus one - فلورسانس منهای یکFlow cytometry - فلوسیتومتریB cell - لنفوسیت بیPneumococcal polysaccharide - پلی ساکارید پنوموکوکPneumococcus - پنوموکوکPolysaccharide - چندقندی ها یا پلیساکاریدها
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Pneumococcal polysaccharide vaccines have been used to elicit a protective anti-pneumococcal polysaccharide antibody response against Streptococcus pneumoniae in healthy individuals. Identifying human B cells which respond to T-cell independent type-2 antigens, such as pneumococcal polysaccharides, has been challenging. We employed pneumococcal polysaccharides directly conjugated to fluorophores in conjunction with flow cytometry to identify the phenotype of B cells that respond to pneumococcal polysaccharide vaccination. We have previously identified that the majority of pneumococcal polysaccharide-selected cells responding to vaccination are CD27+IgM+ (IgM+ memory) cells. In this study, we further characterized pneumococcal polysaccharide-selected cells in the peripheral blood to better identify how the various B cell phenotypes responded 7 and 30 days post-immunization. We show that 7 days post-immunization the majority of pneumococcal polysaccharide-selected IgM+ memory cells (PPS14+ 56.5%, PPS23F+ 63.8%) were CD19+CD20+CD27+IgM+CD43+CD5+/âCD70â, which was significantly increased compared to pre-immunization levels. This phenotype is in alignment with recent publications describing human B-1 cells. PPS-responsive B cells receded to pre-immunization levels by day-30. These findings suggest that this B-1 like cell population plays an important role in early responses to S. pneumoniae infection and possibly other T-cell independent type-2 antigens in humans
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 31, Issue 41, 23 September 2013, Pages 4632-4640
Journal: Vaccine - Volume 31, Issue 41, 23 September 2013, Pages 4632-4640
نویسندگان
David J. Leggat, Noor M. Khaskhely, Anita S. Iyer, Jason Mosakowski, Rebecca S. Thompson, John D. Weinandy, M.A. Julie Westerink,