کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10967251 | 1102816 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A dual vaccine candidate against norovirus and hepatitis E virus
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Norovirus (NoV) and hepatitis E virus (HEV) are both enterically-transmitted viruses causing gastroenteritis and hepatitis, respectively, in humans. While a vaccine against HEVs recently became available in China, there is no prophylactic or therapeutic approach against NoVs. Both NoV and HEV have surface protrusions formed by dimers of the protruding (P) domains of the viral capsids, which is responsible for virus-host interactions and eliciting viral neutralizing antibody. We developed in this study a bivalent vaccine against the two viruses through a recently developed polyvalent complex platform. The dimeric P domains of NoV and HEV were fused together, designated as NoV Pâ-HEV P, which was then linked with the dimeric glutathione-S-transferase (GST). After expression and purification in E. coli, the GST-NoV Pâ-HEV P fusion protein assembled into polyvalent complexes with a mean size of 1.8 μm, while the NoV Pâ-HEV P formed oligomers ranging from 100 to 420 kDa. Mouse immunization study demonstrated that both GST-NoV Pâ-HEV P and NoV Pâ-HEV P complexes induced significantly higher antibody titers to NoV Pâ and HEV P, respectively, than those induced by a mixture of the NoV Pâ and HEV P dimers. Furthermore, the complex-induced antisera exhibited significantly higher neutralizing activity against HEV infection in HepG2/3A cells and higher blocking activity on NoV P particles binding to HBGA receptors than those of the dimer-induced antisera. Thus, GST-NoV Pâ-HEV P and NoV Pâ-HEV P complexes are promising dual vaccine candidates against both NoV and HEV.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 32, Issue 4, 16 January 2014, Pages 445-452
Journal: Vaccine - Volume 32, Issue 4, 16 January 2014, Pages 445-452
نویسندگان
Leyi Wang, Dianjun Cao, Chao Wei, Xiang-Jin Meng, Xi Jiang, Ming Tan,