Control of SIV infection and subsequent induction of pandemic H1N1 immunity in rhesus macaques using an Ad5 [E1-, E2b-] vector platform

► Immunization with Ad5 [E1-, E2b-] results in antigen specific immune induction in Ad5 immune NHP. ► Vaccination with Ad5 [E1-, E2b-]-SIV resulted in significant viral control as compared to controls. ► Immune responses were induced to a second pathogen, H1N1, in the hyper Ad5 immune NHP. ► Ad5 [E1-, E2b-] platform is effective regardless of pre-existing and concomitant vector immunity.