Heterologous prime-boost regimen adenovector 35-circumsporozoite protein vaccine/recombinant Bacillus Calmette-Guérin expressing the Plasmodium falciparum circumsporozoite induces enhanced long-term memory immunity in BALB/c mice

► The heterologous prime-boost regimen Ad35-CS/BCG-CS induces stronger type 1 CSp-specific IFN-γ-producing cells. ► The heterologous prime-boost regimen Ad35-CS/BCG-CS induces higher levels of cytophilic IgG2a. ► The heterologous prime-boost regimen Ad35-CS/BCG-CS regimen elicits the highest level of LLPC-mediated immune responses.