Production of a conjugate vaccine for Salmonella enterica serovar Typhi from Citrobacter Vi

► A new process for Vi purification using a Citrobacter isolate as source. ► Vi conjugation to CRM197 as carrier protein optimized for scale-up. ► The entire process results robust, efficient and affordable. ► Process already used for GMP production of ViCRM197 currently in clinical trials. ► Analytical methods developed to fully characterize the glycoconjugate vaccine.