A pestivirus DNA vaccine based on a non-antibiotic resistance Escherichia coli essential gene marker

► The fabI/triclosan system is a non-antibiotic resistance selection for plasmid DNA. ► The fabI/triclosan system does not compromise immunogenicity. ► The fabI/triclosan selection system displayed high-yield plasmid DNA production. ► The fabI/triclosan system requires only low levels of triclosan for selection (1 μM).