Increased efficacy of an adenovirus-vectored foot-and-mouth disease capsid subunit vaccine expressing nonstructural protein 2B is associated with a specific T cell response

► We constructed Ad5-O1C vectors to examine their efficacy against FMDV in bovines. ► Ad5-O1C construct with full length viral 2B coding region was most efficacious. ► The enhanced protected correlated with an enhanced T cell response.