کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10968179 | 1102873 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid and accurate determination of the potency of varicella vaccine by quantitative polymerase chain reaction
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
⺠The potency of varicella vaccines is currently determined by a plaque assay, which is laborious and has considerable inter- and intra-assay variability. ⺠The increasing demand for varicella vaccine requires a rapid, reliable assay to facilitate the availability of the vaccine. ⺠A new potency assay for varicella vaccine based on quantitative polymerase chain reaction in conjunction with a much more efficient virus infection step is described. ⺠The new assay is much faster and possesses high-throughput capacity in addition to acceptable accuracy and reproducibility when compared with the plaque assay. ⺠Using 23 vaccine lots from 7 manufacturers, we found no significant difference in infectivity between the new assay and plaque assay, suggesting that the assay could potentially be a viable alternative for vaccine analyses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 29, Issue 47, 3 November 2011, Pages 8490-8495
Journal: Vaccine - Volume 29, Issue 47, 3 November 2011, Pages 8490-8495
نویسندگان
Marsha S. Russell, Changgui Li, Louise Larocque, Junzhi Wang, Aaron Farnsworth, Runtao He, Xuguang Li,