Construction and immunogenicity of recombinant adenovirus expressing ORF2 of PCV2 and porcine IFN gamma

► We constructed and characterized the recombinant adenovirus rAd-ORF2-IFN-γ. ► Recombinant induce better ORF2-specific antibody response than rAd-ORF2 alone or adenovirus itself in animal experiment. ► Porcine IFN gamma could strongly enhance the immunogenicity of ORF2. ► Recombinant adenovirus might be an attractive candidate vaccine for preventing PCV2 infection.