Oral vaccination with vaccinia virus expressing the tick antigen subolesin inhibits tick feeding and transmission of Borrelia burgdorferi

► A tick antigen, subolesin, can be expressed from vaccinia virus and is immunogenic. ► Vaccination with subolesin reduced acquisition of Borrelia burdorferi by ticks. ► Vaccination with subolesin reduced transmission of B. burgdorferi by ticks to mice.