A single point mutation in framework region 3 of heavy chain affects viral neutralization dynamics of single-chain Fv against bovine herpes virus type 1

► Construction of single chain Fv that neutralizes bovine herpes virus type 1. ► Single point replacement mutation in FR-3 of scFv affects viral neutralization. ► Dimerization of scFv enhances viral neutralization. ► The scFv provides an antibody based therapeutic and immunodiagnostic protein.