Protection against the toxic effects of Loxosceles intermedia spider venom elicited by mimotope peptides

► Investigation of the reactivity of a monoclonal antibody against continuous peptides from the dermonecrotic protein from Loxosceles intermedia (LiD1). ► Identification of four LiD1 peptide mimotopes using a phage-display approach. ► The putative epitope region (residues C197, Y224, W225, T226, D228, K229, R230, T232 and Y248 of LiD1) recognized by LimAb7 were predicted using MIMOP, a bioinformatic tool. ► Neutralization of the biological effects induced by Loxosceles intermedia spider venom by antibodies anti-mimotope peptide. ► Synthetic immunogens may be promising candidates for alternative therapeutic serum development.