Live attenuated rubella viral vectors stably express HIV and SIV vaccine antigens while reaching high titers

► We created live rubella vectors expressing HIV/SIV antigens in the non-structural gene region. ► Inserts up to 267 bp were stably expressed for at least seven passages. ► They were expressed as early antigens under control of the genomic promoter. ► The vectors, based on the rubella vaccine strain, reached high titers in cell culture.