Eliciting broad neutralizing antibody to HIV-1: Envelopes of different lentivirus cross immunization by prime-boost vaccination

► HIV-1 CN54 gp140 DNA prime plus protein boost was superior to only CN54 gp140 protein immunization. ► HIV-1 CN54 and other species lentivirus cross immunization generate broad and potency Nab. ► CD4+Tem elicited by HIV-1 and SIV cross immune strategy was efficient. ► The Nab elicited by cross immunization aimed at conformational epitopes.