Vaccination with Clostridium perfringens recombinant proteins in combination with Montanide™ ISA 71 VG adjuvant increases protection against experimental necrotic enteritis in commercial broiler chickens

► Birds immunized with NetB or PFO plus ISA 71 exhibited increased body weight gains. ► Greater NetB toxin antibody titers were observed in the NetB/ISA 71 VG group. ► IL-8 gene transcript levels in IELs were reduced in birds immunized with the complex. ► The adjuvant-vaccine combination enhanced protective immunity against NE disease.