Higher cross-subtype IFN-γ ELISpot responses to Gag and Nef peptides in Brazilian HIV-1 subtype B- and F1- than in C-infected subjects

► Isolate sequence most similar to consensus is a useful strategy to immunogen design. ► Subtype B and F1 present a high cross-reactivity T cell response. ► Subtype F1 infected subjects presented higher responses than B- and C-infected subjects.