A totally synthetic lipopeptide-based self-adjuvanting vaccine induces neutralizing antibodies against heat-stable enterotoxin from enterotoxigenic Escherichia coli

► An anti-diarrheal vaccine based on heat-stable toxin (ST). ► Vaccine efficacy is affected by chemical linkage between ST and T helper epitope. ► Geometric orientation of ST determines ability to induce neutralizing antibodies. ► Inoculation by the mucosal route induces strongest neutralizing antibody titers.