کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10968665 | 1102899 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
GP120-specific exosome-targeted T cell-based vaccine capable of stimulating DC- and CD4+ T-independent CTL responses
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
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چکیده انگلیسی
The limitations of highly active anti-retroviral therapy (HAART) have necessitated the development of alternative therapeutics. In this study, we generated ovalbumin (OVA)-pulsed and pcDNAgp120-transfected dendritic cell (DC)-released exosomes (EXOova and EXOgp120) and ConA-stimulated C57BL/6 CD8+ T cells. OVA- and Gp120-Texo vaccines were generated from CD8+ T cells with uptake of EXOova and EXOgp120, respectively. We demonstrate that OVA-Texo stimulates in vitro and in vivo OVA-specific CD4+ and CD8+ cytotoxic T lymphocyte (CTL) responses leading to long-term immunity against OVA-expressing BL6-10OVA melanoma. Interestingly, CD8+ T cell responses are DC and CD4+ T cell independent. Importantly, Gp120-Texo also stimulates Gp120-specific CTL responses and long-term immunity against Gp120-expressing B16 melanoma. Therefore, this novel HIV-1-specific EXO-targeted Gp120-Texo vaccine may be useful in induction of efficient CTL responses in AIDS patients with DC dysfunction and CD4+ T cell deficiency.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 29, Issue 19, 27 April 2011, Pages 3538-3547
Journal: Vaccine - Volume 29, Issue 19, 27 April 2011, Pages 3538-3547
نویسندگان
Roopa Hebbandi Nanjundappa, Rong Wang, Yufeng Xie, Channakeshava Sokke Umeshappa, Rajni Chibbar, Yangdou Wei, Qiang Liu, Jim Xiang,